Concentration and Purification of Poliovirus by Ultrafiltration and Isopycnic Centrifugation
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چکیده
منابع مشابه
Concentration and purification of poliovirus by ultrafiltration and isopycnic centrifugation.
A method is described by which poliovirus can be rapidly and simply concentrated by the use of a Diaflo XM-50 ultrafilter membrane. Freon-extracted ultrafilter concentrates banded in CsCl provided a 1,724-fold volumetric concentration of poliovirus. During concentration, trypsin-digested cellular material can pass through the ultrafilter membrane, thus providing a versatile means of degrading a...
متن کاملIsopycnic centrifugation in ionic media.
Fritsch, A. (1973) Anal. Biochem. 35,57-71 Griffith, 0. M. (1978) Anal. Biochem. 9 0 , 4 3 5 4 4 3 Hames, B. D. & Rickwood, D. (1978) in Centr&gation-A Practical Approach (Rickwood, D., ed.), pp. 69-80, Information Retrieval. London Ridge, D. (1978) in Centrifugal Separations in Molecular and Cell Biology (Birnie, G. D. & Rickwood, D., eds.), pp. 33-61. Butterworths, London Young, B. D. (1978) ...
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The isolated, formaldehyde-fixed nucleoid of E. coli has been analyzed by isopycnic centrifugation in CsCl density gradients. The membrane-free nucleoid bands at a density of 1.69 +/- 0.02 g/cm3. The membrane-associated nucleoid bands at a density of 1.46 +/- 0.02 g/cm3. Both species sediment to equilibrium as nearly monodisperse bands in CsCl, suggesting that the nucleoid components of DNA, RN...
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Solutions of Renografin (30-60%) can be centrifuged to form density gradients in the range from 1.0 g/cm(3) to 1.4 g/cm(3) or, alternatively, preformed gradients can be made which under appropriate conditions of centrifugation have an indefinite stability. Such solutions have a low viscosity and a relatively low ionic strength. The density of DNA in such solutions is surprisingly low ( approxim...
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The re-isolation of trace anlOunts of virus added to tissue hOInogenates has been taken as a Inodel probleIll in the developIllent of high-resolution Illethods for separating particles. FroIll a review of data on these particles, it was found that, when sediIllentation rates are plotted against their banding or buoyant densities, IllOst viI'uses fall in a "window" which appears to be free of ot...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1972
ISSN: 0003-6919
DOI: 10.1128/am.24.1.13-17.1972